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rabbit monoclonal anti-darpp32  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti-darpp32
    Rabbit Monoclonal Anti Darpp32, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+darpp+32/DARPP-32+Rabbit+mAb/pmc12876317-5-0-7
    Average 95 stars, based on 222 article reviews
    rabbit monoclonal anti-darpp32 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Proteomic analysis of X-linked dystonia parkinsonism disease striatal neurons reveals altered RNA metabolism and splicing
    Article Snippet: The following drugs were used: SB-590885 (Tocris, 2650, at 3 μM in DMSO), prostratin (MilliporeSigma, 60857–08–1) at 1 μM in DMSO, and HDACi inhibitor (5 μM in DMSO) ( ). .. To evaluate the effect of drugs treatment on MSNs generation, the cells were fixed using 4% paraformaldehyde (Sigma, 158127) for 30 min and then stained using rabbit anti-DARPP-32 (Cell Signaling, 2306S, 1:200). .. The secondary antibody was donkey anti-rabbit IgG conjugated with Alexa-647 (Invitrogen, A-31573).

    Blocking Assay:

    Article Title: Proteomic analysis of X-linked dystonia parkinsonism disease striatal neurons reveals altered RNA metabolism and splicing
    Article Snippet: .. Secondary antibodies (1:500) were added after three PBS washes in the blocking buffer at RT for 1 h. The following primary antibodies were used for the immunofluorescence studies: rabbit anti-SOX2 (Cell Signaling, 3579S, 1:200), rabbit anti-PAX6 (Biolegend, 19013, 1:100), rabbit anti-NESTIN (Abcam, ab92391, 1:100), rabbit anti-MAP2 (Millipore, AB5622, 1:100), rabbit anti-GABA (Sigma, A2052, 1:100), rabbit anti-DARPP-32 (Cell Signaling, 2306S, 1:200), rabbit anti-TAF1 (Sigma, HPA001075) and rabbit anti-TUBB3 (Cell Signaling, 5568, 1:100). .. Secondary antibodies were donkey anti-rabbit and anti-mouse IgG, conjugated with Alexa-546 (Invitrogen, A10040 and A10036) or Alexa-647 (Invitrogen, A-31573 and A-31571).

    Immunofluorescence:

    Article Title: Proteomic analysis of X-linked dystonia parkinsonism disease striatal neurons reveals altered RNA metabolism and splicing
    Article Snippet: .. Secondary antibodies (1:500) were added after three PBS washes in the blocking buffer at RT for 1 h. The following primary antibodies were used for the immunofluorescence studies: rabbit anti-SOX2 (Cell Signaling, 3579S, 1:200), rabbit anti-PAX6 (Biolegend, 19013, 1:100), rabbit anti-NESTIN (Abcam, ab92391, 1:100), rabbit anti-MAP2 (Millipore, AB5622, 1:100), rabbit anti-GABA (Sigma, A2052, 1:100), rabbit anti-DARPP-32 (Cell Signaling, 2306S, 1:200), rabbit anti-TAF1 (Sigma, HPA001075) and rabbit anti-TUBB3 (Cell Signaling, 5568, 1:100). .. Secondary antibodies were donkey anti-rabbit and anti-mouse IgG, conjugated with Alexa-546 (Invitrogen, A10040 and A10036) or Alexa-647 (Invitrogen, A-31573 and A-31571).

    other:

    Article Title: Parkinson’s-Linked Synaptojanin 1 Loss in Dopamine Neurons Triggers Synaptic Degeneration and Striatal TH Interneuron Compensation
    Article Snippet: The antibodies used in this study were obtained from commercial sources as stated: mouse anti-α-synuclein (610786, RRID: AB_2748880), mouse anti-AP2 (611350, RRID: AB_398872) and mouse anti-Dynamin (610245, RRID: AB_397640) from BD Biosciences; goat anti-ChAT (AB144P, RRID: AB_2079751), mouse anti-clathrin light chain (AB9884, RRID: AB_992745), rat anti-DAT (MAB369, RRID: AB_2190413), mouse anti-SV2C (MABN367, RRID: AB_2905667) and rabbit anti-TH (AB152, RRID: AB_390204) from Merck Millipore; mouse anti-β-actin (sc-47778, RRID: AB_2714189) and mouse anti-BIN1 99D (sc-13575, RRID:AB_626753) from Santa Cruz Biotechnology; rabbit anti-ALDH1A1 (HPA002123, RRID: AB_1844722), rabbit anti-GFAP (ZRB2383, RRID: AB_2905668) and rabbit anti-SJ1 (HPA011916, RRID: AB_1857692) from Sigma-Aldrich; rabbit anti-Endophilin 1 (159002, RID: AB_887757), mouse-anti Calretinin (214111, RRID: AB_2619904), mouse anti-TH (213211, RRID: AB_2636901), rabbit anti-Amphiphysin 1 (120002, RRID: AB_887690), rabbit anti-AADC (369003, RRID: AB_2620131), guinea pig-anti Calbindin D28k (214005, RRID: AB_2619902), mouse anti-Parvalbumin (195011, RRID: AB_2619883) and rabbit anti-D2R (376 203, RRID: AB_2636918) from Synaptic Systems; rabbit anti-VMAT2 (MSFR106400, RRID: AB_2571857) from Nittobo Medical; rabbit anti-DARPP-32 (2306, RRID: AB_823479) and rabbit anti-Neuropeptide Y (11976, RRID: AB_2716286) from Cell Signaling Technology; rabbit anti-Iba1 (019-19741, RRID: AB_839504) from FUJIFILM Wako Pure Chemical Corporation; goat anti-ALDH1A1 (AF5869_RRID: AB_2044597) from R&D Systems; rabbit anti-alpha synuclein phospho S129 (EP1536Y, RRID:AB_765074) from abcam; mouse anti-GAPDH (60004-1, RRID: AB_2107436) and rabbit anti-GAPDH (10494-1-AP, RRID: AB_2263076) from Proteintech.



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